DOT Drug Testing Services

National Drug Testing Network has DOT drug testing and DOT breath alcohol testing centers in all states and most cities near you. These centers are compliant of all the requirements of DOT 49 CFR Part 40. Our process is Fast and Easy! Most of our centers are strategically located within minutes from your home or office.

No appointment is necessary but you must call ahead of time to register for a DOT Drug Test or DOT Breath Alcohol Test.

Call us now to register!

When you need a DOT Drug test or DOT breath alcohol test for employment, random or post accident purposes, you can count on National Drug Testing Network to assist you.


Our DOT Drug Testing Services cover:

  • Truck drivers- CDL operators
  • Boat Captains – Officers
  • Train Engineers
  • Airplane Mechanics
  • Airplane pilots
  • Flight attendants
  • Pipeline workers

If you or your company operate trucks or vehicles with a gross weight rating of 26,001 or more pounds.

If you or your company transport hazardous chemicals


If you or your company operate vehicles which can transport 16 or more passengers. including the driver,

Then you are subject to DOT Drug and Alcohol Testing Regulations!

What will happen if one of my employees tested positive in a DOT drug testing and alcohol test?

If you or any of your employees are required by the Department of Transportation, or if you hold a sensitive designation in your company, then you need to fully comply with Part 40 of the DOT regulations and the office of drug and alcohol policy compliance.

Requirements included are pre-employment drug testing, random drug testing, post-accident drug testing and reasonable suspicion drug testing. Failure to comply gives your employer an authority to remove you from your position. They may also face fines and other penalties.

Your employer is required to immediately remove you from performing safety sensitive functions until you have completed the following:

  • Undergone an evaluation by a Substance Abuse Professional (SAP). Your employer must provide you with a list of SAPs that you can use;
  • Successfully completed any course, counseling or treatment prescribed by the SAP prior to returning to service;
  • Undergone a follow up evaluation by the same SAP to determine your compliance with their recommendations; AND
  • Provided a breath and/or urine specimen that tests negative for drugs and/or alcohol prior to returning to DOT regulated safety-sensitive functions.


   You will also be subject to unannounced “Follow-Up” testing for drugs and/or alcohol for at least 6 times during the first 12 months of active service with the possibility of unannounced testing for up to 60 months (as prescribed by the SAP).  The Return-to-Duty and Follow-Up drug tests will be conducted under direct observation.

Failing to take a random drug or alcohol test once selected can result in substantial fines and penalties by the Department of Transportation (DOT).

National Drug Testing Network provides a DOT approved random selection Consortium for covered employees who are required to comply with DOT random testing requirements.

DOT Post Accident Drug and Alcohol Testing

It is a requirement by the Department of Transportation that any covered employee proceed to take  a drug and breath alcohol test should they encounter an accident that falls under the DOT post accident drug and alcohol testing requirements.

You can contact National Drug Testing Network for you to immediately take the test. We can  conduct the drug and alcohol test at a testing center near the accident area. However, on many occasions a post accident drug and alcohol test will require an on-site/mobile drug testing service which can be provided by National Drug Testing Network as well.

Medical Marijuana Related to DOT

DOJ guidelines will have no bearing on the Department of Transportation’s regulated drug testing program. We will not change our regulated drug testing program based upon these guidelines to Federal prosecutors.

National Drug Testing Network specializes in the development and implementation of a DOT Drug & Alcohol Policy –  Call National Drug Testing Network 888-811-5384

Additional DOT Drug Testing Information

The Department of Transportation only recognizes and authorizes a 5 panel urine drug test and a breath alcohol test for compliance with 49 CFR Part 40.

The staff of National Drug Testing Network are certified and trained in DOT drug specimen collections and DOT breath alcohol testing.

National Drug Testing Network provides DOT physicals nationwide at most drug testing centers and our affiliated medical facilities. All DOT Physicals are administered by an examiner trained and certified by the FMCSA.

National Drug Testing Network is your “one stop shopping” for all DOT Drug Testing Information and DOT Breath Alcohol Testing.

Select Your Test Below

Why Use NDN?

  • Certified and Trained Staff
  • Testing Centers Nationwide
  • Same Day Service Available
  • DOT Certified Staff
  • Court Approved
  • SAMHSA Certified Laboratories
  • Medical Review Officer Verification
  • On-Site / Mobile Testing Available
  • Urine - Hair - Saliva - Blood
  • Friendly and Professional Staff
  • Scheduling Office Open Late and Saturdays
  • 24 hour Online Scheduling Available